Hotspot

accD-psaI

LSC · 58931-59605 · 675 bp

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Pi 0.0228

MarkerSeek score 65.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 31
pi: 0.0228
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	927-943 bp	0.666667	70.6
accD-psaI_p2	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	976-992 bp	0.666667	70.3
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1097-1113 bp	0.666667	70.0
accD-psaI_p4	ACCCGGTTGATTGAGTATGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1167-1183 bp	0.666667	69.6
accD-psaI_p5	TTGATCCGGGTACTTGGAGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1419 bp	0.166667	46.0