Hotspot

trnT-UGU-trnL-UAA

LSC · 46525-47205 · 681 bp

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Pi 0.0172
MarkerSeek score 60.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
681
variable_sites
27
pi
0.0172
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 TTTTCTGGCGATACGAACGT AGCCAATTAAGTCCGTAGCG 45.0 50.0 57.9 58.1 1418-1458 bp 1.0 84.8
trnT-UGU-trnL-UAA_p2 AAAACCTGACCTGTTGACCC AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.9 58.1 1444-1484 bp 1.0 84.7
trnT-UGU-trnL-UAA_p3 ATCTCGGCCGAACAAATCAA AGCCAATTAAGTCCGTAGCG 45.0 50.0 57.9 58.1 961-990 bp 1.0 83.7
trnT-UGU-trnL-UAA_p4 AAAACCTGACCTGTTGACCC GGATTGGAGTTTCGACGGAA 50.0 50.0 57.9 57.9 1634-1669 bp 0.833333 77.9
trnT-UGU-trnL-UAA_p5 TTTTCTGGCGATACGAACGT GGATTGGAGTTTCGACGGAA 45.0 50.0 57.9 57.9 1608-1643 bp 0.833333 77.9