Hotspot

rps4-trnT-UGU

LSC · 46035-46451 · 417 bp

Back to Anabasis

Pi 0.025

MarkerSeek score 66.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 24
pi: 0.025
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTTTGAAACGAGGCCCTC	ATGGTCATCGGTTCGATTCC	50.0	50.0	55.2	57.8	466-506 bp	1.0	48.0
rps4-trnT-UGU_p2	TTTTGAAACGAGGCCCTC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	55.2	57.9	498-538 bp	1.0	47.7
rps4-trnT-UGU_p3	TTTTGAAACGAGGCCCTC	AGGTTAGAGCATCGCATTTGT	50.0	42.9	55.2	58.0	493-533 bp	1.0	47.7
rps4-trnT-UGU_p4	TTTTGAAACGAGGCCCTC	CAGAGGTTAGAGCATCGCAT	50.0	50.0	55.2	57.5	496-536 bp	1.0	47.6
rps4-trnT-UGU_p5	TTTTGAAACGAGGCCCTC	TCAGAGGTTAGAGCATCGCA	50.0	50.0	55.2	58.5	497-537 bp	1.0	47.6