Hotspot

rpoB-trnC-GCA

LSC · 26371-27476 · 1106 bp

Back to Anabasis

Pi 0.0152
MarkerSeek score 60.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1106
variable_sites
39
pi
0.0152
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGAGCCCATATCCTTGCTTT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1664-1693 bp 1.0 83.8
rpoB-trnC-GCA_p2 ACCCTCATTTCCATCCCGTA GGAATGGAGAGGTCTCGGTA 50.0 55.0 58.1 57.9 1386-1419 bp 0.833333 77.7
rpoB-trnC-GCA_p3 TACCCTCATTTCCATCCCGT GGAATGGAGAGGTCTCGGTA 50.0 55.0 58.1 57.9 1387-1420 bp 0.833333 77.7
rpoB-trnC-GCA_p4 CGAGCCCATATCCTTGCTTT GGAATGGAGAGGTCTCGGTA 50.0 55.0 58.0 57.9 1874-1907 bp 0.833333 77.1
rpoB-trnC-GCA_p5 CCTCATTTCCATCCCGTAGC GGAATGGAGAGGTCTCGGTA 55.0 55.0 58.1 57.9 1384-1417 bp 0.666667 70.4