Hotspot

trnK-UUU-rps16

LSC · 4174-4922 · 749 bp

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Pi 0.0255

MarkerSeek score 66.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 42
pi: 0.0255
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	TCTATCCCAATGAGCCGTCT	55.0	50.0	58.0	57.9	1025-1086 bp	1.0	86.6
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AGCCGTCTATCGAATCGTTG	55.0	50.0	58.0	57.9	1013-1074 bp	1.0	86.4
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	1021-1082 bp	1.0	86.2
trnK-UUU-rps16_p4	TTCAGGATCAGTCGTGGTCT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	1022-1083 bp	1.0	86.2
trnK-UUU-rps16_p5	CGGGTAAATTCATCAGCGGA	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	1530-1594 bp	0.666667	71.8