Hotspot

rps7-trnL-UUG

IRa · 111134-112338 · 1205 bp

Back to Amyema

Pi 0.054
MarkerSeek score 84.80
Primer available yes
Label rps7-trnL-UUG

Feature Metadata

label_name
rps7-trnL-UUG
feature_type
igs
region
IRa
length_bp
1205
variable_sites
109
pi
0.054
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-trnL-UUG_p1 ATGGCAGAGGCAAATAGAGC GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 568-1276 bp 1.0 91.7
rps7-trnL-UUG_p2 ATGGCAGAGGCAAATAGAGC CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 566-1274 bp 1.0 91.7
rps7-trnL-UUG_p3 GGCAGAGGCAAATAGAGCTT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.0 58.0 566-1274 bp 1.0 91.4
rps7-trnL-UUG_p4 GGCAGAGGCAAATAGAGCTT CTCGCGTGTCTACCATTTCA 50.0 50.0 58.0 58.0 564-1272 bp 1.0 91.4
rps7-trnL-UUG_p5 ATGGCAGAGGCAAATAGAGC TACCATTTCACCACCAAGGC 50.0 50.0 58.0 58.1 556-1264 bp 1.0 91.1