Hotspot

ccsA-psaC

SSC · 94717-95057 · 341 bp

Back to Amyema

Pi 0.11
MarkerSeek score 77.40
Primer available yes
Label ccsA-psaC

Feature Metadata

label_name
ccsA-psaC
feature_type
igs
region
SSC
length_bp
341
variable_sites
73
pi
0.11
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psaC_p1 TGCAGCTCTTTTATGCGGAT CATGTATCGGGTGCACTCAA 45.0 50.0 58.0 58.0 1076-1095 bp 1.0 91.5
ccsA-psaC_p2 TGCAGCTCTTTTATGCGGAT TGTATCGGGTGCACTCAATG 45.0 50.0 58.0 58.0 1074-1093 bp 1.0 91.5
ccsA-psaC_p3 GCATGGGGCTCATATTGGAA CATGTATCGGGTGCACTCAA 50.0 50.0 58.0 58.0 772-787 bp 0.8 83.8
ccsA-psaC_p4 GCATGGGGCTCATATTGGAA TGTATCGGGTGCACTCAATG 50.0 50.0 58.0 58.0 770-785 bp 0.8 83.8
ccsA-psaC_p5 GCATGGGGCTCATATTGGAA GTATCGGGTGCACTCAATGT 50.0 50.0 58.0 58.0 769-784 bp 0.8 83.8