Hotspot

rpl36-rps8

LSC · 66622-67155 · 534 bp

Back to Amyema

Pi 0.0579
MarkerSeek score 80.90
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
534
variable_sites
60
pi
0.0579
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 AATGCTGAATCTCTGCCGAG GCTGCAGTTGTCATTGATCC 50.0 50.0 58.0 57.4 1502-1816 bp 1.0 85.4
rpl36-rps8_p2 ATTCGTCGTCGTTTTCGGAT AATGACAGACCGAGAAGCTC 45.0 50.0 58.0 57.3 617-701 bp 0.8 76.9
rpl36-rps8_p3 ATTCGTCGTCGTTTTCGGAT GCTGCAGTTGTCATTGATCC 45.0 50.0 58.0 57.4 1236-1308 bp 0.8 76.3
rpl36-rps8_p4 CAGCAAAAGGTGTCCCTCTT GCTGCAGTTGTCATTGATCC 50.0 50.0 58.0 57.4 1665-1713 bp 0.8 75.9
rpl36-rps8_p5 CAGCAAAAGGTGTCCCTCTT AATGACAGACCGAGAAGCTC 50.0 50.0 58.0 57.3 1047-1106 bp 0.8 75.7