Hotspot

ycf4-cemA

LSC · 49266-49366 · 101 bp

Back to Amyema

Pi 0.0693
MarkerSeek score 79.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
101
variable_sites
15
pi
0.0693
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 AAAAGTCGCGGAATTAGCCT AGGAATTCCTGAACCCGAGA 45.0 50.0 57.9 58.0 584-611 bp 0.8 75.1
ycf4-cemA_p2 GTGCTAGTTGATGGGCGTTA AGGAATTCCTGAACCCGAGA 50.0 50.0 58.0 58.0 1186-1213 bp 0.8 74.5
ycf4-cemA_p3 TGCTAGTTGATGGGCGTTAC AGGAATTCCTGAACCCGAGA 50.0 50.0 58.0 58.0 1185-1212 bp 0.6 64.9
ycf4-cemA_p4 GTGCTAGTTGATGGGCGTTA TGAGAGAGGGATCCACCAAG 50.0 55.0 58.0 58.1 839-866 bp 0.6 64.3
ycf4-cemA_p5 TGCTAGTTGATGGGCGTTAC TGAGAGAGGGATCCACCAAG 50.0 55.0 58.0 58.1 838-865 bp 0.6 64.3