Hotspot

trnL-UUA-trnF-UUC

LSC · 40915-41123 · 209 bp

Back to Amyema

Pi 0.0708
MarkerSeek score 80.40
Primer available yes
Label trnL-UUA-trnF-UUC

Feature Metadata

label_name
trnL-UUA-trnF-UUC
feature_type
igs
region
LSC
length_bp
209
variable_sites
31
pi
0.0708
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UUA-trnF-UUC_p1 TTCAAGTCCCTCTATCCCCA TACCAACTGAGCTATCCCGA 50.0 50.0 57.0 57.6 242-253 bp 1.0 80.7
trnL-UUA-trnF-UUC_p2 ATCATGAGGGTTCAAGTCCC TACCAACTGAGCTATCCCGA 50.0 50.0 56.9 57.6 252-263 bp 1.0 79.5
trnL-UUA-trnF-UUC_p3 TTCAAGTCCCTCTATCCCCA TCAGTCCTCTGCTCTACCAA 50.0 50.0 57.0 57.4 256-267 bp 1.0 79.5
trnL-UUA-trnF-UUC_p4 TTCAAGTCCCTCTATCCCCA TCCTCTGCTCTACCAACTGA 50.0 50.0 57.0 57.4 252-263 bp 1.0 79.5
trnL-UUA-trnF-UUC_p5 TTCAAGTCCCTCTATCCCCA TTCAGTCCTCTGCTCTACCA 50.0 50.0 57.0 57.4 257-268 bp 1.0 79.5