Hotspot

rps4-trnT-ACA

LSC · 39664-40096 · 433 bp

Back to Amyema

Pi 0.106
MarkerSeek score 82.40
Primer available yes
Label rps4-trnT-ACA

Feature Metadata

label_name
rps4-trnT-ACA
feature_type
igs
region
LSC
length_bp
433
variable_sites
83
pi
0.106
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-ACA_p1 TTTTTAAAACGAGGCCCTC CTCAGAGGTTAGAGCATCGC 42.1 55.0 53.5 57.9 483-704 bp 1.0 52.0
rps4-trnT-ACA_p2 TTTTTAAAACGAGGCCCTC ATGGTCATCGGTTCGATTCC 42.1 50.0 53.5 57.8 451-672 bp 1.0 52.0
rps4-trnT-ACA_p3 TTTTTAAAACGAGGCCCTC CCGCTTAGCTCAGAGGTTAG 42.1 55.0 53.5 57.5 491-712 bp 1.0 52.0
rps4-trnT-ACA_p4 TTTTTAAAACGAGGCCCTC CAGAGGTTAGAGCATCGCAT 42.1 50.0 53.5 57.5 481-702 bp 1.0 52.0
rps4-trnT-ACA_p5 TTTTTAAAACGAGGCCCTC TCAGAGGTTAGAGCATCGCA 42.1 50.0 53.5 58.5 482-703 bp 1.0 52.0