Hotspot

ycf3-trnS-UCC

LSC · 38170-38793 · 624 bp

Back to Amyema

Pi 0.0464
MarkerSeek score 88.90
Primer available yes
Label ycf3-trnS-UCC

Feature Metadata

label_name
ycf3-trnS-UCC
feature_type
igs
region
LSC
length_bp
624
variable_sites
66
pi
0.0464
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
88.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-UCC_p1 GGCTTCCCTTTCTCTTGAAGT TTCCAATGCTACGCCTTGAA 47.6 45.0 57.9 57.8 767-866 bp 1.0 79.8
ycf3-trnS-UCC_p2 AGGCTTCCCTTTCTCTTGAAG TTCCAATGCTACGCCTTGAA 47.6 45.0 57.6 57.8 768-867 bp 1.0 77.6
ycf3-trnS-UCC_p3 GGCTTCCCTTTCTCTTGAAGT CCCTCGGTAAACAAAAGCCT 47.6 50.0 57.9 58.1 796-895 bp 0.8 72.0
ycf3-trnS-UCC_p4 AGGCTTCCCTTTCTCTTGAAG CCCTCGGTAAACAAAAGCCT 47.6 50.0 57.6 58.1 797-896 bp 0.8 69.7
ycf3-trnS-UCC_p5 GGCTTCCCTTTCTCTTGAAGT ACCCTCGGTAAACAAAAGCC 47.6 50.0 57.9 58.4 797-896 bp 0.8 69.7