Hotspot

trnE-GAA-trnT-ACC

LSC · 25001-25563 · 563 bp

Back to Amyema

Pi 0.0835
MarkerSeek score 84.10
Primer available yes
Label trnE-GAA-trnT-ACC

Feature Metadata

label_name
trnE-GAA-trnT-ACC
feature_type
igs
region
LSC
length_bp
563
variable_sites
89
pi
0.0835
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-GAA-trnT-ACC_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 517-696 bp 1.0 89.8
trnE-GAA-trnT-ACC_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 496-675 bp 1.0 89.7
trnE-GAA-trnT-ACC_p3 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 514-693 bp 1.0 88.7
trnE-GAA-trnT-ACC_p4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 50.0 55.0 57.7 58.2 493-672 bp 1.0 88.5
trnE-GAA-trnT-ACC_p5 GCTGCCTCCTTGAAAGAGAG TTGAACCGATGACTTACGCC 55.0 50.0 58.3 58.3 519-698 bp 1.0 87.6