Hotspot

trnD-GAC-trnY-UAC

LSC · 24462-24787 · 326 bp

Back to Amyema

Pi 0.049
MarkerSeek score 80.10
Primer available yes
Label trnD-GAC-trnY-UAC

Feature Metadata

label_name
trnD-GAC-trnY-UAC
feature_type
igs
region
LSC
length_bp
326
variable_sites
35
pi
0.049
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GAC-trnY-UAC_p1 CGGTGCTCTAACCAGTTGAA TCTACGCTGGTTCAAATCCG 50.0 50.0 57.8 58.0 386-423 bp 1.0 88.3
trnD-GAC-trnY-UAC_p2 GGTGCTCTAACCAGTTGAACT TCTACGCTGGTTCAAATCCG 47.6 50.0 57.9 58.0 385-422 bp 1.0 80.7
trnD-GAC-trnY-UAC_p3 CGGTGCTCTAACCAGTTGAA CTACGCTGGTTCAAATCCGG 50.0 55.0 57.8 59.0 385-422 bp 1.0 80.5
trnD-GAC-trnY-UAC_p4 CGGTGCTCTAACCAGTTGAA TACGCTGGTTCAAATCCGG 50.0 52.6 57.8 57.9 384-421 bp 1.0 79.3
trnD-GAC-trnY-UAC_p5 CGGTGCTCTAACCAGTTGAA TGTCTACGCTGGTTCAAATCC 50.0 47.6 57.8 58.3 388-425 bp 1.0 77.9