Hotspot

psbM-trnD-GAC

LSC · 23683-24387 · 705 bp

Back to Amyema

Pi 0.0393
MarkerSeek score 83.20
Primer available yes
Label psbM-trnD-GAC

Feature Metadata

label_name
psbM-trnD-GAC
feature_type
igs
region
LSC
length_bp
705
variable_sites
59
pi
0.0393
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GAC_p1 TTGACTAGCCGTTTTTACGT TTCAACTGGTTAGAGCACCG 40.0 50.0 55.4 57.8 721-871 bp 1.0 67.2
psbM-trnD-GAC_p2 AGCCGTTTTTACGTAAAGGA TTCAACTGGTTAGAGCACCG 40.0 50.0 55.3 57.8 715-865 bp 1.0 67.0
psbM-trnD-GAC_p3 TTTGACTAGCCGTTTTTACGT TTCAACTGGTTAGAGCACCG 38.1 50.0 56.1 57.8 722-872 bp 1.0 65.5
psbM-trnD-GAC_p4 AGCCGTTTTTACGTAAAGGAT TTCAACTGGTTAGAGCACCG 38.1 50.0 55.9 57.8 715-865 bp 1.0 63.6
psbM-trnD-GAC_p5 ACTAGCCGTTTTTACGTAAAGGA TTCAACTGGTTAGAGCACCG 39.1 50.0 58.1 57.8 718-868 bp 1.0 63.5