Hotspot

petN-psbM

LSC · 22831-23568 · 738 bp

Back to Amyema

Pi 0.0416
MarkerSeek score 84.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
738
variable_sites
62
pi
0.0416
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATCGCTTGGGCTGCTTTAAT ACGTAAAAACGGCTAGTCAA 45.0 40.0 58.2 55.4 663-881 bp 1.0 67.0
petN-psbM_p2 ATCGCTTGGGCTGCTTTAAT TCCTTTACGTAAAAACGGCT 45.0 40.0 58.2 55.3 669-887 bp 1.0 66.7
petN-psbM_p3 GTATCGCTTGGGCTGCTTTA ACGTAAAAACGGCTAGTCAA 50.0 40.0 58.3 55.4 665-883 bp 1.0 66.1
petN-psbM_p4 GTATCGCTTGGGCTGCTTTA TCCTTTACGTAAAAACGGCT 50.0 40.0 58.3 55.3 671-889 bp 1.0 65.8
petN-psbM_p5 TATCGCTTGGGCTGCTTTAA ACGTAAAAACGGCTAGTCAA 45.0 40.0 57.6 55.4 664-882 bp 1.0 65.5