Hotspot

psbK-psbI

LSC · 4166-4594 · 429 bp

Back to Amyema

Pi 0.156
MarkerSeek score 78.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
429
variable_sites
126
pi
0.156
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CTTACCACTTGACTACGCCC TAAAGCGGCGGATTGCTAAT 55.0 45.0 58.0 58.0 1118-1252 bp 1.0 91.6
psbK-psbI_p2 GACTACGCCCCAATTCGATT TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.1 58.0 1108-1242 bp 1.0 91.3
psbK-psbI_p3 CTTACCACTTGACTACGCCC TCCGTTGTACGAGTTATGCG 55.0 50.0 58.0 58.1 1099-1233 bp 1.0 91.1
psbK-psbI_p4 CTTACCACTTGACTACGCCC TGGACCTATTCGGGCTGTAT 55.0 50.0 58.0 57.9 1260-1394 bp 0.8 83.2
psbK-psbI_p5 CACTCGCTTGTAAGGGGTTT TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.1 58.0 1376-1437 bp 0.6 74.8