primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbK-psbI_p1 psbK-psbI 1 CTTACCACTTGACTACGCCC TAAAGCGGCGGATTGCTAAT 20 20 55.0 45.0 58.0 58.0 0.0 9.8 0.044177 4166 4594 1118-1252 1.000000 91.6 psbK-psbI_p2 psbK-psbI 2 GACTACGCCCCAATTCGATT TAAAGCGGCGGATTGCTAAT 20 20 50.0 45.0 58.1 58.0 0.0 9.8 0.088741 4166 4594 1108-1242 1.000000 91.3 psbK-psbI_p3 psbK-psbI 3 CTTACCACTTGACTACGCCC TCCGTTGTACGAGTTATGCG 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.107032 4166 4594 1099-1233 1.000000 91.1 psbK-psbI_p4 psbK-psbI 4 CTTACCACTTGACTACGCCC TGGACCTATTCGGGCTGTAT 20 20 55.0 50.0 58.0 57.9 0.0 0.0 0.103081 4166 4594 1260-1394 0.800000 83.2 psbK-psbI_p5 psbK-psbI 5 CACTCGCTTGTAAGGGGTTT TAAAGCGGCGGATTGCTAAT 20 20 50.0 45.0 58.1 58.0 8.5 9.8 0.145997 4166 4594 1376-1437 0.600000 74.8 trnC-UGC-petN_p1 trnC-UGC-petN 1 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.314369 22446 22731 300-396 1.000000 89.5 trnC-UGC-petN_p2 trnC-UGC-petN 2 GTATCATTTTGGCGGCATGG ATTAAAGCAGCCCAAGCGAT 20 20 50.0 45.0 57.9 58.2 14.3 0.0 0.370837 22446 22731 352-448 1.000000 89.0 trnC-UGC-petN_p3 trnC-UGC-petN 3 TTTCCCCAGTTCAAATCCGG TAAAGCAGCCCAAGCGATAC 20 20 50.0 50.0 58.1 58.3 0.0 0.0 0.418433 22446 22731 298-394 1.000000 88.7 trnC-UGC-petN_p4 trnC-UGC-petN 4 GTATCATTTTGGCGGCATGG TAAAGCAGCCCAAGCGATAC 20 20 50.0 50.0 57.9 58.3 14.3 0.0 0.474902 22446 22731 350-446 1.000000 88.2 trnC-UGC-petN_p5 trnC-UGC-petN 5 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCGATA 20 20 50.0 45.0 58.1 57.6 0.0 0.0 0.493756 22446 22731 299-395 1.000000 88.0 petN-psbM_p1 petN-psbM 1 ATCGCTTGGGCTGCTTTAAT ACGTAAAAACGGCTAGTCAA 20 20 45.0 40.0 58.2 55.4 0.0 2.8 2.881970 22831 23568 663-881 1.000000 67.0 petN-psbM_p2 petN-psbM 2 ATCGCTTGGGCTGCTTTAAT TCCTTTACGTAAAAACGGCT 20 20 45.0 40.0 58.2 55.3 0.0 20.5 2.913057 22831 23568 669-887 1.000000 66.7 petN-psbM_p3 petN-psbM 3 GTATCGCTTGGGCTGCTTTA ACGTAAAAACGGCTAGTCAA 20 20 50.0 40.0 58.3 55.4 0.0 2.8 2.986034 22831 23568 665-883 1.000000 66.1 petN-psbM_p4 petN-psbM 4 GTATCGCTTGGGCTGCTTTA TCCTTTACGTAAAAACGGCT 20 20 50.0 40.0 58.3 55.3 0.0 20.5 3.017121 22831 23568 671-889 1.000000 65.8 petN-psbM_p5 petN-psbM 5 TATCGCTTGGGCTGCTTTAA ACGTAAAAACGGCTAGTCAA 20 20 45.0 40.0 57.6 55.4 0.0 2.8 3.061356 22831 23568 664-882 1.000000 65.5 psbM-trnD-GAC_p1 psbM-trnD-GAC 1 TTGACTAGCCGTTTTTACGT TTCAACTGGTTAGAGCACCG 20 20 40.0 50.0 55.4 57.8 0.0 0.0 2.813547 23683 24387 721-871 1.000000 67.2 psbM-trnD-GAC_p2 psbM-trnD-GAC 2 AGCCGTTTTTACGTAAAGGA TTCAACTGGTTAGAGCACCG 20 20 40.0 50.0 55.3 57.8 22.4 0.0 2.844634 23683 24387 715-865 1.000000 67.0 psbM-trnD-GAC_p3 psbM-trnD-GAC 3 TTTGACTAGCCGTTTTTACGT TTCAACTGGTTAGAGCACCG 21 20 38.1 50.0 56.1 57.8 0.0 0.0 3.024094 23683 24387 722-872 1.000000 65.5 psbM-trnD-GAC_p4 psbM-trnD-GAC 4 AGCCGTTTTTACGTAAAGGAT TTCAACTGGTTAGAGCACCG 21 20 38.1 50.0 55.9 57.8 22.4 0.0 3.269050 23683 24387 715-865 1.000000 63.6 psbM-trnD-GAC_p5 psbM-trnD-GAC 5 ACTAGCCGTTTTTACGTAAAGGA TTCAACTGGTTAGAGCACCG 23 20 39.1 50.0 58.1 57.8 22.4 0.0 3.281898 23683 24387 718-868 1.000000 63.5 trnD-GAC-trnY-UAC_p1 trnD-GAC-trnY-UAC 1 CGGTGCTCTAACCAGTTGAA TCTACGCTGGTTCAAATCCG 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.168401 24462 24787 386-423 1.000000 88.3 trnD-GAC-trnY-UAC_p2 trnD-GAC-trnY-UAC 2 GGTGCTCTAACCAGTTGAACT TCTACGCTGGTTCAAATCCG 21 20 47.6 50.0 57.9 58.0 0.0 0.0 1.132008 24462 24787 385-422 1.000000 80.7 trnD-GAC-trnY-UAC_p3 trnD-GAC-trnY-UAC 3 CGGTGCTCTAACCAGTTGAA CTACGCTGGTTCAAATCCGG 20 20 50.0 55.0 57.8 59.0 0.0 0.0 1.156986 24462 24787 385-422 1.000000 80.5 trnD-GAC-trnY-UAC_p4 trnD-GAC-trnY-UAC 4 CGGTGCTCTAACCAGTTGAA TACGCTGGTTCAAATCCGG 20 19 50.0 52.6 57.8 57.9 0.0 0.0 1.310601 24462 24787 384-421 1.000000 79.3 trnD-GAC-trnY-UAC_p5 trnD-GAC-trnY-UAC 5 CGGTGCTCTAACCAGTTGAA TGTCTACGCTGGTTCAAATCC 20 21 50.0 47.6 57.8 58.3 0.0 0.0 1.468459 24462 24787 388-425 1.000000 77.9 trnE-GAA-trnT-ACC_p1 trnE-GAA-trnT-ACC 1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 20 20 55.0 50.0 58.3 58.0 0.0 0.0 0.269359 25001 25563 517-696 1.000000 89.8 trnE-GAA-trnT-ACC_p2 trnE-GAA-trnT-ACC 2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.287509 25001 25563 496-675 1.000000 89.7 trnE-GAA-trnT-ACC_p3 trnE-GAA-trnT-ACC 3 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 20 20 55.0 55.0 58.3 58.2 0.0 0.0 0.414292 25001 25563 514-693 1.000000 88.7 trnE-GAA-trnT-ACC_p4 trnE-GAA-trnT-ACC 4 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 20 20 50.0 55.0 57.7 58.2 0.0 0.0 0.432442 25001 25563 493-672 1.000000 88.5 trnE-GAA-trnT-ACC_p5 trnE-GAA-trnT-ACC 5 GCTGCCTCCTTGAAAGAGAG TTGAACCGATGACTTACGCC 20 20 55.0 50.0 58.3 58.3 0.0 0.0 0.546195 25001 25563 519-698 1.000000 87.6 trnS-UCA-psbZ_p1 trnS-UCA-psbZ 1 CAACCACTCAGCCATCTCTC GCAAAAACAGCTAATTGGAAAGC 20 23 55.0 39.1 58.0 58.0 0.0 0.0 3.043690 29555 29913 412-677 1.000000 66.3 trnS-UCA-psbZ_p2 trnS-UCA-psbZ 2 GAGCTATCAACCACTCAGCC GCAAAAACAGCTAATTGGAAAGC 20 23 55.0 39.1 58.1 58.0 0.0 0.0 3.075590 29555 29913 419-684 1.000000 65.9 trnS-UCA-psbZ_p3 trnS-UCA-psbZ 3 GGAGCTATCAACCACTCAGC GCAAAAACAGCTAATTGGAAAGC 20 23 55.0 39.1 58.1 58.0 0.0 0.0 3.075590 29555 29913 420-685 1.000000 65.9 trnS-UCA-psbZ_p4 trnS-UCA-psbZ 4 CAAGACCGGAGCTATCAACC GCAAAAACAGCTAATTGGAAAGC 20 23 55.0 39.1 58.1 58.0 9.5 0.0 3.081010 29555 29913 427-692 1.000000 65.8 trnS-UCA-psbZ_p5 trnS-UCA-psbZ 5 CGGAGCTATCAACCACTCAG GCAAAAACAGCTAATTGGAAAGC 20 23 55.0 39.1 57.8 58.0 0.0 0.0 3.238242 29555 29913 421-686 1.000000 64.6 ycf3-trnS-UCC_p1 ycf3-trnS-UCC 1 GGCTTCCCTTTCTCTTGAAGT TTCCAATGCTACGCCTTGAA 21 20 47.6 45.0 57.9 57.8 0.0 5.6 1.340217 38170 38793 767-866 1.000000 79.8 ycf3-trnS-UCC_p2 ycf3-trnS-UCC 2 AGGCTTCCCTTTCTCTTGAAG TTCCAATGCTACGCCTTGAA 21 20 47.6 45.0 57.6 57.8 0.0 5.6 1.624336 38170 38793 768-867 1.000000 77.6 ycf3-trnS-UCC_p3 ycf3-trnS-UCC 3 GGCTTCCCTTTCTCTTGAAGT CCCTCGGTAAACAAAAGCCT 21 20 47.6 50.0 57.9 58.1 0.0 0.0 1.241916 38170 38793 796-895 0.800000 72.0 ycf3-trnS-UCC_p4 ycf3-trnS-UCC 4 AGGCTTCCCTTTCTCTTGAAG CCCTCGGTAAACAAAAGCCT 21 20 47.6 50.0 57.6 58.1 0.0 0.0 1.526035 38170 38793 797-896 0.800000 69.7 ycf3-trnS-UCC_p5 ycf3-trnS-UCC 5 GGCTTCCCTTTCTCTTGAAGT ACCCTCGGTAAACAAAAGCC 21 20 47.6 50.0 57.9 58.4 0.0 0.0 1.534877 38170 38793 797-896 0.800000 69.7 rps4-trnT-ACA_p1 rps4-trnT-ACA 1 TTTTTAAAACGAGGCCCTC CTCAGAGGTTAGAGCATCGC 19 20 42.1 55.0 53.5 57.9 0.0 0.0 5.666820 39664 40096 483-704 1.000000 52.0 rps4-trnT-ACA_p2 rps4-trnT-ACA 2 TTTTTAAAACGAGGCCCTC ATGGTCATCGGTTCGATTCC 19 20 42.1 50.0 53.5 57.8 0.0 0.0 5.774574 39664 40096 451-672 1.000000 52.0 rps4-trnT-ACA_p3 rps4-trnT-ACA 3 TTTTTAAAACGAGGCCCTC CCGCTTAGCTCAGAGGTTAG 19 20 42.1 55.0 53.5 57.5 0.0 7.6 6.034589 39664 40096 491-712 1.000000 52.0 rps4-trnT-ACA_p4 rps4-trnT-ACA 4 TTTTTAAAACGAGGCCCTC CAGAGGTTAGAGCATCGCAT 19 20 42.1 50.0 53.5 57.5 0.0 0.0 6.056916 39664 40096 481-702 1.000000 52.0 rps4-trnT-ACA_p5 rps4-trnT-ACA 5 TTTTTAAAACGAGGCCCTC TCAGAGGTTAGAGCATCGCA 19 20 42.1 50.0 53.5 58.5 0.0 0.0 6.059751 39664 40096 482-703 1.000000 52.0 trnL-UUA-trnF-UUC_p1 trnL-UUA-trnF-UUC 1 TTCAAGTCCCTCTATCCCCA TACCAACTGAGCTATCCCGA 20 20 50.0 50.0 57.0 57.6 0.0 0.0 1.408944 40915 41123 242-253 1.000000 80.7 trnL-UUA-trnF-UUC_p2 trnL-UUA-trnF-UUC 2 ATCATGAGGGTTCAAGTCCC TACCAACTGAGCTATCCCGA 20 20 50.0 50.0 56.9 57.6 0.1 0.0 1.557895 40915 41123 252-263 1.000000 79.5 trnL-UUA-trnF-UUC_p3 trnL-UUA-trnF-UUC 3 TTCAAGTCCCTCTATCCCCA TCAGTCCTCTGCTCTACCAA 20 20 50.0 50.0 57.0 57.4 0.0 0.0 1.566448 40915 41123 256-267 1.000000 79.5 trnL-UUA-trnF-UUC_p4 trnL-UUA-trnF-UUC 4 TTCAAGTCCCTCTATCCCCA TCCTCTGCTCTACCAACTGA 20 20 50.0 50.0 57.0 57.4 0.0 0.0 1.566448 40915 41123 252-263 1.000000 79.5 trnL-UUA-trnF-UUC_p5 trnL-UUA-trnF-UUC 5 TTCAAGTCCCTCTATCCCCA TTCAGTCCTCTGCTCTACCA 20 20 50.0 50.0 57.0 57.4 0.0 0.0 1.566448 40915 41123 257-268 1.000000 79.5 ycf4-cemA_p1 ycf4-cemA 1 AAAAGTCGCGGAATTAGCCT AGGAATTCCTGAACCCGAGA 20 20 45.0 50.0 57.9 58.0 6.1 26.5 0.156803 49266 49366 584-611 0.800000 75.1 ycf4-cemA_p2 ycf4-cemA 2 GTGCTAGTTGATGGGCGTTA AGGAATTCCTGAACCCGAGA 20 20 50.0 50.0 58.0 58.0 0.0 26.5 0.065921 49266 49366 1186-1213 0.800000 74.5 ycf4-cemA_p3 ycf4-cemA 3 TGCTAGTTGATGGGCGTTAC AGGAATTCCTGAACCCGAGA 20 20 50.0 50.0 58.0 58.0 0.0 26.5 0.065921 49266 49366 1185-1212 0.600000 64.9 ycf4-cemA_p4 ycf4-cemA 4 GTGCTAGTTGATGGGCGTTA TGAGAGAGGGATCCACCAAG 20 20 50.0 55.0 58.0 58.1 0.0 15.2 0.147124 49266 49366 839-866 0.600000 64.3 ycf4-cemA_p5 ycf4-cemA 5 TGCTAGTTGATGGGCGTTAC TGAGAGAGGGATCCACCAAG 20 20 50.0 55.0 58.0 58.1 0.0 15.2 0.147124 49266 49366 838-865 0.600000 64.3 rpl36-rps8_p1 rpl36-rps8 1 AATGCTGAATCTCTGCCGAG GCTGCAGTTGTCATTGATCC 20 20 50.0 50.0 58.0 57.4 0.0 16.6 0.605706 66622 67155 1502-1816 1.000000 85.4 rpl36-rps8_p2 rpl36-rps8 2 ATTCGTCGTCGTTTTCGGAT AATGACAGACCGAGAAGCTC 20 20 45.0 50.0 58.0 57.3 1.3 0.0 0.677128 66622 67155 617-701 0.800000 76.9 rpl36-rps8_p3 rpl36-rps8 3 ATTCGTCGTCGTTTTCGGAT GCTGCAGTTGTCATTGATCC 20 20 45.0 50.0 58.0 57.4 1.3 16.6 0.569836 66622 67155 1236-1308 0.800000 76.3 rpl36-rps8_p4 rpl36-rps8 4 CAGCAAAAGGTGTCCCTCTT GCTGCAGTTGTCATTGATCC 20 20 50.0 50.0 58.0 57.4 0.0 16.6 0.569652 66622 67155 1665-1713 0.800000 75.9 rpl36-rps8_p5 rpl36-rps8 5 CAGCAAAAGGTGTCCCTCTT AATGACAGACCGAGAAGCTC 20 20 50.0 50.0 58.0 57.3 0.0 0.0 0.676944 66622 67155 1047-1106 0.800000 75.7 trnL-UUG-rps7_p1 trnL-UUG-rps7 1 GTCTCGCGTGTCTACCATTT ATGGCAGAGGCAAATAGAGC 20 20 50.0 50.0 58.0 58.0 5.8 0.0 0.029376 81026 82230 568-1276 1.000000 91.6 trnL-UUG-rps7_p2 trnL-UUG-rps7 2 CTCGCGTGTCTACCATTTCA ATGGCAGAGGCAAATAGAGC 20 20 50.0 50.0 58.0 58.0 5.8 0.0 0.030746 81026 82230 566-1274 1.000000 91.6 trnL-UUG-rps7_p3 trnL-UUG-rps7 3 GTCTCGCGTGTCTACCATTT GGCAGAGGCAAATAGAGCTT 20 20 50.0 50.0 58.0 58.0 5.8 0.0 0.057719 81026 82230 566-1274 1.000000 91.4 trnL-UUG-rps7_p4 trnL-UUG-rps7 4 CTCGCGTGTCTACCATTTCA GGCAGAGGCAAATAGAGCTT 20 20 50.0 50.0 58.0 58.0 5.8 0.0 0.059089 81026 82230 564-1272 1.000000 91.4 trnL-UUG-rps7_p5 trnL-UUG-rps7 5 TACCATTTCACCACCAAGGC ATGGCAGAGGCAAATAGAGC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.103674 81026 82230 556-1264 1.000000 91.1 trnL-CUA-ccsA_p1 trnL-CUA-ccsA 1 ACGCTGTTCTTAGGAAGCAG ATCCGCATAAAAGAGCTGCA 20 20 50.0 45.0 57.9 58.0 10.7 1.0 0.128740 93659 93756 609-628 1.000000 87.2 trnL-CUA-ccsA_p2 trnL-CUA-ccsA 2 ACGCTGTTCTTAGGAAGCAG TCCGCATAAAAGAGCTGCAT 20 20 50.0 45.0 57.9 58.0 10.7 1.0 0.128740 93659 93756 608-627 1.000000 87.2 trnL-CUA-ccsA_p3 trnL-CUA-ccsA 3 CGCTGTTCTTAGGAAGCAGT ATCCGCATAAAAGAGCTGCA 20 20 50.0 45.0 57.9 58.0 14.2 1.0 0.128740 93659 93756 608-627 1.000000 87.2 trnL-CUA-ccsA_p4 trnL-CUA-ccsA 4 CGCTGTTCTTAGGAAGCAGT TCCGCATAAAAGAGCTGCAT 20 20 50.0 45.0 57.9 58.0 14.2 1.0 0.128740 93659 93756 607-626 1.000000 87.2 trnL-CUA-ccsA_p5 trnL-CUA-ccsA 5 CCGAGAAGAAGGTATCAGCG TCCGCATAAAAGAGCTGCAT 20 20 55.0 45.0 57.9 58.0 0.0 1.0 0.169600 93659 93756 1277-1299 0.800000 73.0 ccsA-psaC_p1 ccsA-psaC 1 TGCAGCTCTTTTATGCGGAT CATGTATCGGGTGCACTCAA 20 20 45.0 50.0 58.0 58.0 0.0 18.7 0.065450 94717 95057 1076-1095 1.000000 91.5 ccsA-psaC_p2 ccsA-psaC 2 TGCAGCTCTTTTATGCGGAT TGTATCGGGTGCACTCAATG 20 20 45.0 50.0 58.0 58.0 0.0 18.7 0.065450 94717 95057 1074-1093 1.000000 91.5 ccsA-psaC_p3 ccsA-psaC 3 GCATGGGGCTCATATTGGAA CATGTATCGGGTGCACTCAA 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.023116 94717 95057 772-787 0.800000 83.8 ccsA-psaC_p4 ccsA-psaC 4 GCATGGGGCTCATATTGGAA TGTATCGGGTGCACTCAATG 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.023116 94717 95057 770-785 0.800000 83.8 ccsA-psaC_p5 ccsA-psaC 5 GCATGGGGCTCATATTGGAA GTATCGGGTGCACTCAATGT 20 20 50.0 50.0 58.0 58.0 0.0 18.7 0.024527 94717 95057 769-784 0.800000 83.8 psaC-ycf1_p1 psaC-ycf1 1 CACATTGAGTGCACCCGATA TGAACCGATTCGTCTGTCTG 20 20 50.0 50.0 58.0 57.7 14.6 5.2 0.370462 95304 95727 825-948 1.000000 89.0 psaC-ycf1_p2 psaC-ycf1 2 CATTGAGTGCACCCGATACA TGAACCGATTCGTCTGTCTG 20 20 50.0 50.0 58.0 57.7 14.6 5.2 0.370462 95304 95727 823-946 1.000000 89.0 psaC-ycf1_p3 psaC-ycf1 3 TTGAGTGCACCCGATACATG TGAACCGATTCGTCTGTCTG 20 20 50.0 50.0 58.0 57.7 14.6 5.2 0.370462 95304 95727 821-944 1.000000 89.0 psaC-ycf1_p4 psaC-ycf1 4 ACATTGAGTGCACCCGATAC TGAACCGATTCGTCTGTCTG 20 20 50.0 50.0 58.0 57.7 14.6 5.2 0.371873 95304 95727 824-947 1.000000 89.0 psaC-ycf1_p5 psaC-ycf1 5 TTGAGTGCACCCGATACATG GAACCGATTCGTCTGTCTGT 20 20 50.0 50.0 58.0 57.7 14.6 5.2 0.372062 95304 95727 820-943 1.000000 89.0 rps7-trnL-UUG_p1 rps7-trnL-UUG 1 ATGGCAGAGGCAAATAGAGC GTCTCGCGTGTCTACCATTT 20 20 50.0 50.0 58.0 58.0 0.0 5.8 0.029376 111134 112338 568-1276 1.000000 91.7 rps7-trnL-UUG_p2 rps7-trnL-UUG 2 ATGGCAGAGGCAAATAGAGC CTCGCGTGTCTACCATTTCA 20 20 50.0 50.0 58.0 58.0 0.0 5.8 0.030746 111134 112338 566-1274 1.000000 91.7 rps7-trnL-UUG_p3 rps7-trnL-UUG 3 GGCAGAGGCAAATAGAGCTT GTCTCGCGTGTCTACCATTT 20 20 50.0 50.0 58.0 58.0 0.0 5.8 0.057719 111134 112338 566-1274 1.000000 91.4 rps7-trnL-UUG_p4 rps7-trnL-UUG 4 GGCAGAGGCAAATAGAGCTT CTCGCGTGTCTACCATTTCA 20 20 50.0 50.0 58.0 58.0 0.0 5.8 0.059089 111134 112338 564-1272 1.000000 91.4 rps7-trnL-UUG_p5 rps7-trnL-UUG 5 ATGGCAGAGGCAAATAGAGC TACCATTTCACCACCAAGGC 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.103674 111134 112338 556-1264 1.000000 91.1