Hotspot

petD

LSC · 77715-78929 · 1215 bp

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Pi 0.00911

MarkerSeek score 49.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1215
variable_sites: 11
pi: 0.00911
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTCCTTACCCTGGGACCAA	CAGAATTGCCTCCCAGGATC	50.0	55.0	57.9	58.0	2022-2033 bp	1.0	80.3
petD_p2	TCCTTACCCTGGGACCAAAT	CAGAATTGCCTCCCAGGATC	50.0	55.0	57.9	58.0	2020-2031 bp	1.0	80.3
petD_p3	CGTGTGTATCTTACCGGTGG	CAGAATTGCCTCCCAGGATC	55.0	55.0	58.1	58.0	2122-2133 bp	1.0	80.2
petD_p4	ATTCCTTACCCTGGGACCAA	CTTCATGAAGCATCCCGGAA	50.0	50.0	57.9	58.0	2209-2220 bp	1.0	80.1
petD_p5	TCCTTACCCTGGGACCAAAT	CTTCATGAAGCATCCCGGAA	50.0	50.0	57.9	58.0	2207-2218 bp	1.0	80.1