Hotspot

ndhG

SSC · 118633-119163 · 531 bp

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Pi 0.00377

MarkerSeek score 45.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CAAGCTCGGACACATTGAGT	CTGCCCGTTGTAGATTGGAA	50.0	50.0	58.2	57.9	1770-1997 bp	1.0	79.1
ndhG_p2	CAAGCTCGGACACATTGAGT	ACAAACAATACGAGCTGCCA	50.0	45.0	58.2	58.1	1963-2190 bp	1.0	79.0
ndhG_p3	CAAGCTCGGACACATTGAGT	TGTGAAGTATGCGTTCGTGT	50.0	45.0	58.2	57.9	1803-2030 bp	1.0	78.8
ndhG_p4	CAAGCTCGGACACATTGAGT	GCTTGTGAAGTATGCGTTCG	50.0	50.0	58.2	57.9	1806-2033 bp	1.0	78.7
ndhG_p5	CAAGCTCGGACACATTGAGT	ACATCGGTCAGGGTTTTACG	50.0	50.0	58.2	57.9	1940 bp	0.5	57.7