Hotspot

rps7-rps12

IRb · 98712-98764 · 53 bp

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Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TGGATCGGATTTGGCTGTTT	GGCTTGGAGGGAGATCTTTC	45.0	55.0	57.8	57.7	170 bp	1.0	76.0
rps7-rps12_p2	TTGGATCGGATTTGGCTGTT	GGCTTGGAGGGAGATCTTTC	45.0	55.0	57.8	57.7	171 bp	1.0	76.0
rps7-rps12_p3	ATTGGATCGGATTTGGCTGT	GGCTTGGAGGGAGATCTTTC	45.0	55.0	57.6	57.7	172 bp	1.0	74.2
rps7-rps12_p4	TGGATCGGATTTGGCTGTTT	TATGTACGGCTTGGAGGGAG	45.0	55.0	57.8	58.6	177 bp	1.0	74.0
rps7-rps12_p5	TTGGATCGGATTTGGCTGTT	TATGTACGGCTTGGAGGGAG	45.0	55.0	57.8	58.6	178 bp	1.0	74.0