Hotspot

petD-rpoA

LSC · 78930-79123 · 194 bp

Back to Amsonia

Pi 0.0211

MarkerSeek score 66.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 4
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TCGTTCTAGAAAAACATTTGGCA	45.0	34.8	57.9	57.2	295-299 bp	1.0	51.4
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TCGTTCTAGAAAAACATTTGGCA	45.0	34.8	57.9	57.2	296-300 bp	1.0	51.4
petD-rpoA_p3	CCCTTTGGTTGGGTATTGGT	TCGTTCTAGAAAAACATTTGGCA	50.0	34.8	57.7	57.2	299-303 bp	1.0	49.5
petD-rpoA_p4	AGCGACAACCGTCTTTTTGA	TCGTTCTAGAAAAACATTTGGCA	45.0	34.8	58.3	57.2	334-338 bp	1.0	49.1
petD-rpoA_p5	TTGGTTGGGTATTGGTGCAA	GTCGTTCTAGAAAAACATTTGGC	45.0	39.1	57.9	56.9	296-300 bp	1.0	49.0