Hotspot

petA-psbJ

LSC · 64167-65173 · 1007 bp

Back to Amsonia

Pi 0.0295

MarkerSeek score 71.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 29
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1135-1146 bp	1.0	79.6
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1066-1077 bp	1.0	79.6
petA-psbJ_p3	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1068-1079 bp	1.0	79.6
petA-psbJ_p4	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1065-1076 bp	1.0	78.8
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1142-1153 bp	1.0	75.3