Hotspot

trnE-UUC-trnT-GGU

LSC · 31325-32177 · 853 bp

Back to Amsonia

Pi 0.00744
MarkerSeek score 66.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
853
variable_sites
6
pi
0.00744
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGAGATGTCCTGGACCAC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 895-942 bp 1.0 83.3
trnE-UUC-trnT-GGU_p2 GAGAGATGTCCTGGACCACT GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 894-941 bp 1.0 83.3
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 947-994 bp 1.0 83.0
trnE-UUC-trnT-GGU_p4 AGAGAGATGTCCTGGACCAC CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 874-921 bp 1.0 82.9
trnE-UUC-trnT-GGU_p5 GAGAGATGTCCTGGACCACT CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 873-920 bp 1.0 82.9