Hotspot

petN-psbM

LSC · 28842-29982 · 1141 bp

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Pi 0.00649

MarkerSeek score 61.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 7
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1218-1237 bp	1.0	76.2
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1216-1235 bp	1.0	76.2
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1214-1233 bp	1.0	75.6
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1212-1231 bp	1.0	75.6
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	1215-1234 bp	1.0	75.6