Hotspot

rps15-ndhF

IRb · 102104-102211 · 108 bp

Back to Amphipogon

Pi 0.068

MarkerSeek score 74.20

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 108
variable_sites: 7
pi: 0.068
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	GTTTGCTGGCTTATTTGGCA	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	57.5	58.0	316-329 bp	1.0	79.5
rps15-ndhF_p2	ATTGTCGAGCCGAAAGATGG	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.3	58.0	829-842 bp	1.0	78.8
rps15-ndhF_p3	GAGCCGAAAGATGGATGCAT	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.4	58.0	823-836 bp	1.0	78.3
rps15-ndhF_p4	ACGGAGACTTGCTTCACATT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	57.4	58.0	402-415 bp	1.0	78.0
rps15-ndhF_p5	ATTGTCGAGCCGAAAGATGG	TGGAGTGGGTCTTGCTAGTT	50.0	50.0	58.3	58.3	828-841 bp	1.0	77.0