Hotspot

cemA-petA

LSC · 60087-60314 · 228 bp

Back to Amphipogon

Pi 0.0307

MarkerSeek score 69.40

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 228
variable_sites: 7
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CCGTCTATCTCCTTCGCTTG	TCGATCGAATCATCTGTTCCT	55.0	42.9	57.9	56.6	332-334 bp	1.0	61.8
cemA-petA_p2	AACCGTCTATCTCCTTCGCT	TCGATCGAATCATCTGTTCCT	50.0	42.9	58.2	56.6	334-336 bp	1.0	60.9
cemA-petA_p3	ACCGTCTATCTCCTTCGCTT	TCGATCGAATCATCTGTTCCT	50.0	42.9	58.2	56.6	333-335 bp	1.0	60.9
cemA-petA_p4	CCGTCTATCTCCTTCGCTTG	TCGATCGAATCATCTGTTCCTT	55.0	40.9	57.9	57.3	332-334 bp	1.0	59.4
cemA-petA_p5	ACCGTCTATCTCCTTCGCTT	TCGATCGAATCATCTGTTCCTT	50.0	40.9	58.2	57.3	333-335 bp	1.0	58.6