Hotspot

atpI-atpH

LSC · 32825-33629 · 805 bp

Back to Amphipogon

Pi 0.0276

MarkerSeek score 68.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 22
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	897-899 bp	1.0	77.4
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	874-876 bp	1.0	77.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	941-943 bp	1.0	76.9
atpI-atpH_p4	ATTTTTGCAACGTTAGCCGC	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.6	57.9	910-912 bp	1.0	76.8
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	896-898 bp	1.0	76.8