Hotspot

trnD-GUC-psbM

LSC · 16701-17749 · 1049 bp

Back to Amphipogon

Pi 0.0417
MarkerSeek score 61.80
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1049
variable_sites
23
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.53
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCA 50.0 40.0 58.5 54.5 676-1134 bp 1.0 54.1
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG ATGCGAGAATATTGACTTCCA 50.0 38.1 58.5 55.1 677-1135 bp 1.0 51.0
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCAT 50.0 38.1 58.5 55.1 676-1134 bp 1.0 51.0
trnD-GUC-psbM_p4 GTTCAATTGGTCAGAGCACC TGCGAGAATATTGACTTCCA 50.0 40.0 57.0 54.5 677-1135 bp 1.0 49.9
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG AATGCGAGAATATTGACTTCCA 50.0 36.4 58.5 55.9 678-1136 bp 1.0 49.2