Hotspot

trnG-UCC-trnT-GGU

LSC · 13810-15492 · 1683 bp

Back to Amphipogon

Pi 0.0419
MarkerSeek score 75.10
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1683
variable_sites
68
pi
0.0419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1729-1773 bp 1.0 46.6
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1725-1769 bp 1.0 45.9
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1726-1770 bp 1.0 45.9
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1732-1776 bp 1.0 45.9
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1731-1775 bp 1.0 45.9