Hotspot

rps16-trnQ-UUG

LSC · 5626-6762 · 1137 bp

Back to Amphipogon

Pi 0.0309
MarkerSeek score 65.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1137
variable_sites
25
pi
0.0309
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.71
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 883-1211 bp 1.0 84.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTCACTCGGAGGTTCGAATC 45.0 55.0 58.1 58.1 891-1219 bp 1.0 84.1
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 894-1222 bp 1.0 83.6
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GTCACTCGGAGGTTCGAATC 50.0 55.0 58.2 58.1 902-1230 bp 1.0 83.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 887-1215 bp 1.0 83.1