Hotspot

trnK-UUU-rps16

LSC · 3954-4513 · 560 bp

Back to Amphipogon

Pi 0.0403
MarkerSeek score 71.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
560
variable_sites
22
pi
0.0403
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG AGAAAAAGAAAGAGCTTTGAGT 50.0 31.8 58.6 54.1 638 bp 0.5 20.0
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA AGAAAAAGAAAGAGCTTTGAGT 45.0 31.8 57.3 54.1 639 bp 0.5 20.0
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA AGAAAAAGAAAGAGCTTTGAGT 45.0 31.8 57.3 54.1 636 bp 0.5 20.0
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG AGAAAGAAAAAGAAAGAGCTTTGAGT 50.0 30.8 58.6 57.9 642 bp 0.5 20.0
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG AGAAAAAGAAAGAGCTTTGAGTT 50.0 30.4 58.6 54.9 638 bp 0.5 20.0