Hotspot

ndhF

SSC · 113433-115691 · 2259 bp

Back to Amphicarpaea

Pi 0.0146

MarkerSeek score 52.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 33
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TAGGCTTGTGGAAGAAGGGA	AACCGACGAATAAGAAACCA	50.0	40.0	58.0	55.0	2642-2657 bp	1.0	57.3
ndhF_p2	TAGGCTTGTGGAAGAAGGGA	ACCGACGAATAAGAAACCAA	50.0	40.0	58.0	55.0	2641-2656 bp	1.0	57.3
ndhF_p3	CCTTCTTCGAGCTAGGCTTG	AACCGACGAATAAGAAACCA	55.0	40.0	58.1	55.0	2654-2669 bp	1.0	57.1
ndhF_p4	CCTTCTTCGAGCTAGGCTTG	ACCGACGAATAAGAAACCAA	55.0	40.0	58.1	55.0	2653-2668 bp	1.0	57.1
ndhF_p5	GGGCCCTCTTATCTCTTCCT	ACCGACGAATAAGAAACCAA	55.0	40.0	57.9	55.0	2670-2685 bp	1.0	56.7