Hotspot

accD

LSC · 61394-62905 · 1512 bp

Back to Amphicarpaea

Pi 0.0133

MarkerSeek score 57.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 20
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCTTCATCCAATGACTATACATCCA	TCTAGTGATTATAGCTAAGCCA	36.0	36.4	57.4	53.6	1612-1622 bp	1.0	41.6
accD_p2	TCTTCATCCAATGACTATACATCCA	TCTAGTGATTATAGCTAAGCCAA	36.0	34.8	57.4	54.4	1612-1622 bp	1.0	41.6
accD_p3	TCTTCATCCAATGACTATACATCCA	TTCTAGTGATTATAGCTAAGCCA	36.0	34.8	57.4	54.4	1613-1623 bp	1.0	41.6
accD_p4	TCATCCAATGACTATACATCCA	TCTAGTGATTATAGCTAAGCCA	36.4	36.4	54.1	53.6	1609-1619 bp	1.0	41.6
accD_p5	TCTTCATCCAATGACTATACATCCA	AGTGATTATAGCTAAGCCAAA	36.0	33.3	57.4	52.3	1609-1619 bp	1.0	41.6