Hotspot

psbK-trnQ-UUG

LSC · 58705-59175 · 471 bp

Back to Amphicarpaea

Pi 0.0195

MarkerSeek score 67.00

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 9
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	GGATTCAAGAAAGCGTAGGC	GATATGCTCTGGGACGGAAG	50.0	55.0	56.9	57.6	646-655 bp	1.0	69.8
psbK-trnQ-UUG_p2	TAGGCTTCGGGCAATTTCG	GATATGCTCTGGGACGGAAG	52.6	55.0	58.2	57.6	631-640 bp	1.0	69.0
psbK-trnQ-UUG_p3	GGATTCAAGAAAGCGTAGGC	GACCAAAACCCGTTGCCTTA	50.0	50.0	56.9	58.7	604-613 bp	1.0	68.1
psbK-trnQ-UUG_p4	TAGGCTTCGGGCAATTTCG	GACCAAAACCCGTTGCCTTA	52.6	50.0	58.2	58.7	589-598 bp	1.0	67.3
psbK-trnQ-UUG_p5	GGATTCAAGAAAGCGTAGGC	AATGGATATGCTCTGGGACG	50.0	50.0	56.9	57.2	650-659 bp	1.0	66.6