Hotspot

trnT-GGU-trnE-UUC

LSC · 33666-34486 · 821 bp

Back to Amphicarpaea

Pi 0.0186

MarkerSeek score 63.00

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 15
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	AACTTCCTTTCGTAGCACCC	50.0	50.0	58.0	57.8	956-969 bp	1.0	80.9
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	AACTTCCTTTCGTAGCACCC	55.0	50.0	58.1	57.8	935-948 bp	1.0	80.5
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	911-924 bp	1.0	80.4
trnT-GGU-trnE-UUC_p4	TGGCGTTACTCTACCACTGA	AACTTCCTTTCGTAGCACCC	50.0	50.0	58.1	57.8	932-945 bp	1.0	80.3
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	890-903 bp	1.0	80.3