Pi
0.0144
MarkerSeek score
62.80
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 821-836 bp | 1.0 | 82.3 |
| trnL-UAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 829-844 bp | 1.0 | 81.7 |
| trnL-UAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGATTCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 853-868 bp | 1.0 | 81.3 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGATTCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 861-876 bp | 1.0 | 80.7 |
| trnL-UAA-trnT-UGU_p5 | ACCAATTTCGCCATATCCCC | TTGAGAAAAGCCGGCTATCG | 50.0 | 50.0 | 58.0 | 58.3 | 872-887 bp | 1.0 | 80.4 |