Hotspot

rpl2-trnH-GUG

LSC · 3920-4086 · 167 bp

Back to Amphicarpaea

Pi 0.103

MarkerSeek score 63.50

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 4
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.23
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GGGCCCCAATTGGTAGAAAA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.1	58.1	359 bp	0.5	58.5
rpl2-trnH-GUG_p2	GGGCCCCAATTGGTAGAAAA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.1	57.9	345 bp	0.5	58.5
rpl2-trnH-GUG_p3	GGGCCCCAATTGGTAGAAAA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.1	57.9	344 bp	0.5	58.5
rpl2-trnH-GUG_p4	GGGCCCCAATTGGTAGAAAA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.1	57.9	346 bp	0.5	58.5
rpl2-trnH-GUG_p5	AACTCCTTGGGGTTTTCCTG	CGGATGTAGCCAAGTGGATC	50.0	55.0	57.6	58.1	331 bp	0.5	55.9