Hotspot

ndhG

SSC · 125836-126366 · 531 bp

Back to Ampelopsis

Pi 0.000418

MarkerSeek score 26.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.000418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TCATGAGAGAGGGCCAAGAT	ATGATTACCTTGTCCCACGC	50.0	50.0	57.8	58.0	1656-1982 bp	1.0	80.5
ndhG_p2	TCATGAGAGAGGGCCAAGAT	ACAAACAGTACGAGCTGCAA	50.0	45.0	57.8	58.1	1696-2022 bp	1.0	80.3
ndhG_p3	CTTCTGGATCGGCTCATGAG	ATGATTACCTTGTCCCACGC	55.0	50.0	57.8	58.0	1669-1995 bp	0.888889	75.7
ndhG_p4	CTTCTGGATCGGCTCATGAG	AACAAACAGTACGAGCTGCA	55.0	45.0	57.8	58.1	1710-2036 bp	0.888889	75.6
ndhG_p5	CTTCTGGATCGGCTCATGAG	ACAAACAGTACGAGCTGCAA	55.0	45.0	57.8	58.1	1709-2035 bp	0.888889	75.6