Hotspot

ndhA

SSC · 127111-129369 · 2259 bp

Back to Ampelopsis

Pi 0.00236

MarkerSeek score 54.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 20
pi: 0.00236
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	58.1	56.5	2342-2380 bp	1.0	61.2
ndhA_p2	TGAACCCAGTTACCATAGGGA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	57.8	56.5	2345-2383 bp	1.0	60.1
ndhA_p3	ACCCAGTTACCATAGGGAACA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	58.1	57.2	2342-2380 bp	1.0	59.2
ndhA_p4	TGAACCCAGTTACCATAGGGA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	57.8	57.2	2345-2383 bp	1.0	58.1
ndhA_p5	ACCCAGTTACCATAGGGAACA	ATGGGAGAAGTTGATCGTTGA	47.6	42.9	58.1	57.0	2343-2381 bp	1.0	57.4