Hotspot

ndhG-ndhI

SSC · 126367-126539 · 173 bp

Back to Ampelopsis

Pi 0.0474

MarkerSeek score 68.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 173
variable_sites: 23
pi: 0.0474
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	ATGATTACCTTGTCCCACGC	50.0	50.0	58.0	58.0	765-976 bp	1.0	83.6
ndhG-ndhI_p2	GTGCAGCAGCTACAAAATGG	TTGATCACATCGGAGCGTTT	50.0	45.0	58.0	57.9	705-916 bp	1.0	83.5
ndhG-ndhI_p3	GTGCAGCAGCTACAAAATGG	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	805-1016 bp	1.0	83.4
ndhG-ndhI_p4	GTGCAGCAGCTACAAAATGG	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	806-1017 bp	1.0	83.3
ndhG-ndhI_p5	GTGCAGCAGCTACAAAATGG	TACCTTGTCCCACGCAAATC	50.0	50.0	58.0	58.2	760-971 bp	1.0	82.5