Hotspot

rpl32-trnL-UAG

SSC · 120672-121846 · 1175 bp

Back to Ampelopsis

Pi 0.00603

MarkerSeek score 54.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1175
variable_sites: 26
pi: 0.00603
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAAAGGAAGGGATGGTGGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1316-1331 bp	1.0	80.4
rpl32-trnL-UAG_p2	GGAAAAGGAAGGGATGGTGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1317-1332 bp	1.0	80.4
rpl32-trnL-UAG_p3	GAAAAGGAAGGGATGGTGGG	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.1	57.8	1309-1324 bp	1.0	79.9
rpl32-trnL-UAG_p4	GGAAAAGGAAGGGATGGTGG	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.1	57.8	1310-1325 bp	1.0	79.9
rpl32-trnL-UAG_p5	GAAAAGGAAGGGATGGTGGG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.1	57.8	1314-1329 bp	1.0	79.8