Hotspot

psbZ-trnfM-CAU

LSC · 40167-41088 · 922 bp

Back to Ampelopsis

Pi 0.0199
MarkerSeek score 65.50
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
922
variable_sites
66
pi
0.0199
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 TCTGGTAGGTATACTTAATTCTCTCA GTCACGGGTTCAAATCCTGT 34.6 50.0 56.5 58.1 955-1056 bp 1.0 49.4
psbZ-trnfM-CAU_p2 TCTGGTAGGTATACTTAATTCTCTCA TCACGGGTTCAAATCCTGTC 34.6 50.0 56.5 57.8 954-1055 bp 1.0 49.4
psbZ-trnfM-CAU_p3 TCTGGTAGGTATACTTAATTCTCTCA CACGGGTTCAAATCCTGTCT 34.6 50.0 56.5 57.8 953-1054 bp 1.0 49.4
psbZ-trnfM-CAU_p4 TCTGGTAGGTATACTTAATTCTCTCA CTTGAGGTCACGGGTTCAAA 34.6 50.0 56.5 58.0 961-1062 bp 1.0 49.3
psbZ-trnfM-CAU_p5 TCTGGTAGGTATACTTAATTCTCTCA TGAGGTCACGGGTTCAAATC 34.6 50.0 56.5 57.8 959-1060 bp 1.0 49.3