Hotspot

trnS-GCU-trnR-UCU

LSC · 10090-12063 · 1974 bp

Back to Ampelopsis

Pi 0.00393

MarkerSeek score 55.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1974
variable_sites: 30
pi: 0.00393
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	1992-2052 bp	1.0	68.1
trnS-GCU-trnR-UCU_p2	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	1998-2058 bp	1.0	67.4
trnS-GCU-trnR-UCU_p3	GCTTTAGTCCACTCAGCCAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.6	56.9	1978-2038 bp	1.0	65.2
trnS-GCU-trnR-UCU_p4	GCTTTAGTCCACTCAGCCAT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.6	57.9	1984-2044 bp	1.0	64.5
trnS-GCU-trnR-UCU_p5	AGTCCACTCAGCCATCTCTC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.5	56.9	1973-2033 bp	1.0	64.4