Hotspot

trnL-UAA

SSC · 118965-119003 · 39 bp

Back to Ampelocera

Pi 0.047

MarkerSeek score 70.40

Primer available yes

Label trnL-UAA

Feature Metadata

label_name: trnL-UAA
feature_type: tRNA
region: SSC
length_bp: 39
variable_sites: 3
pi: 0.047
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA_p1	TCTTTTGAAAGGGGTCAGTT	AAAGTGTTGGATGTCATTTTT	40.0	28.6	54.7	52.4	621-763 bp	0.75	36.1
trnL-UAA_p2	TTCTTTTGAAAGGGGTCAGT	AAAGTGTTGGATGTCATTTTT	40.0	28.6	54.7	52.4	622-764 bp	0.75	36.1
trnL-UAA_p3	TTTGTGATTTCCCGGTTCTT	AAAGTGTTGGATGTCATTTTT	40.0	28.6	55.5	52.4	644-786 bp	0.75	36.0
trnL-UAA_p4	ATTTGTGATTTCCCGGTTCT	AAAGTGTTGGATGTCATTTTT	40.0	28.6	55.2	52.4	645-787 bp	0.75	36.0
trnL-UAA_p5	ATTTGTGATTTCCCGGTTCTT	AAAGTGTTGGATGTCATTTTT	38.1	28.6	56.0	52.4	645-787 bp	0.75	36.0
trnL-UAA_p1	AAAAACCCACATACGGCGAA	TGCTGCCCAATATCCTTTCC	45.0	50.0	58.4	57.9	1334-1478 bp	1.0	82.3
trnL-UAA_p2	AAAAACCCACATACGGCGAA	GGCTCGTGAAAAGAGTCCAT	45.0	50.0	58.4	57.9	961-1081 bp	1.0	82.2
trnL-UAA_p3	CCCACATACGGCGAAGATTT	TGCTGCCCAATATCCTTTCC	50.0	50.0	58.3	57.9	1329-1473 bp	0.75	72.3
trnL-UAA_p4	CCCACATACGGCGAAGATTT	GGCTCGTGAAAAGAGTCCAT	50.0	50.0	58.3	57.9	956-1076 bp	0.75	72.2
trnL-UAA_p5	CCCACATACGGCGAAGATTT	GTCAGGCTCGTGAAAAGAGT	50.0	50.0	58.3	57.8	960-1080 bp	0.75	71.7