Hotspot

ndhA

SSC · 126745-129340 · 2596 bp

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Pi 0.0146

MarkerSeek score 62.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2596
variable_sites: 61
pi: 0.0146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGATCCAAATTGTGA	GCCCGCCAGGTTTTATCAAT	33.3	50.0	57.0	58.9	2678-2755 bp	1.0	45.8
ndhA_p2	CTCTTGTTTGATCCAAATTGTGA	GCCCGCCAGGTTTTATCAAT	34.8	50.0	55.7	58.9	2677-2754 bp	1.0	45.8
ndhA_p3	TCTCTTGTTTGATCCAAATTGTG	GCCCGCCAGGTTTTATCAAT	34.8	50.0	55.7	58.9	2678-2755 bp	1.0	45.8
ndhA_p4	TCTCTTGTTTGATCCAAATTGTGAA	GCCCGCCAGGTTTTATCAAT	32.0	50.0	57.7	58.9	2678-2755 bp	1.0	45.8
ndhA_p5	TTCTCTTGTTTGATCCAAATTGTGA	GCCCGCCAGGTTTTATCAAT	32.0	50.0	57.7	58.9	2679-2756 bp	1.0	45.8