Hotspot

rpl32-trnL-UAG

SSC · 119717-121019 · 1303 bp

Back to Ampelocera

Pi 0.0211

MarkerSeek score 69.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1303
variable_sites: 47
pi: 0.0211
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAAGGATATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1406-1485 bp	1.0	85.6
rpl32-trnL-UAG_p2	GGAAAGGATATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1407-1486 bp	1.0	85.6
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1394-1473 bp	1.0	84.8
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1395-1474 bp	1.0	84.8
rpl32-trnL-UAG_p5	GGAAAGGATATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1416-1495 bp	1.0	84.6