Hotspot

rbcL-accD

LSC · 61314-62141 · 828 bp

Back to Ampelocera

Pi 0.0298

MarkerSeek score 70.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 45
pi: 0.0298
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCATGGTATGCACTTCCGTG	TGATTTACTTAGCCCGCACC	50.0	50.0	58.0	58.0	1394-1411 bp	1.0	85.1
rbcL-accD_p2	TCATGGTATGCACTTCCGTG	ATTTACTTAGCCCGCACCTG	50.0	50.0	58.0	58.0	1392-1409 bp	1.0	85.1
rbcL-accD_p3	TCATGGTATGCACTTCCGTG	GACCACCAAGACTGTTCGTT	50.0	50.0	58.0	58.1	1413-1430 bp	1.0	84.9
rbcL-accD_p4	TCATGGTATGCACTTCCGTG	CCACCAAGACTGTTCGTTGA	50.0	50.0	58.0	58.1	1411-1428 bp	1.0	84.9
rbcL-accD_p5	ACTGCAAATACTAGCCTGGC	TGATTTACTTAGCCCGCACC	50.0	50.0	58.0	58.0	1489-1506 bp	1.0	84.6