Hotspot

trnT-UGU-trnL-UAA

LSC · 50473-52175 · 1703 bp

Back to Ampelocera

Pi 0.0313
MarkerSeek score 71.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1703
variable_sites
83
pi
0.0313
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 1396-1753 bp 1.0 87.0
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 1427-1784 bp 1.0 86.7
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 1404-1761 bp 1.0 86.5
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GTTTCCATACCAAGGCCCAA 55.0 50.0 57.9 58.1 1438-1795 bp 1.0 86.4
trnT-UGU-trnL-UAA_p5 GAGTCGAACCGATGACCATC GTTTCCATACCAAGGCCCAA 55.0 50.0 58.2 58.1 1469-1826 bp 1.0 86.1