Hotspot

trnG-GCC-trnfM-CAU

LSC · 40087-40260 · 174 bp

Back to Ampelocera

Pi 0.0289
MarkerSeek score 65.60
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
174
variable_sites
9
pi
0.0289
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 237-240 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 238-241 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 235-238 bp 1.0 82.8
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT TGAGGTCACGGGTTCAAATC 50.0 50.0 58.1 57.8 236-239 bp 1.0 82.8
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.1 57.8 257-260 bp 1.0 82.1